Coding

Part:BBa_K3419005:Design

Designed by: Leo Song   Group: iGEM20_Cornell   (2020-10-18)


Holin/Anti-Holin Kill Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1393
    Illegal NheI site found at 1416
    Illegal NheI site found at 1961
    Illegal NheI site found at 1984
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 998
    Illegal AgeI site found at 1068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1649


Design Notes

This kill switch is an improved and adapted version of the kill switch offered by the 2013 iGEM TUDelft team. This kill switch has a degradation tag as well as a lactate inducible promoter to make the bacteria specific to growing in high lactate environments.


Source

http://2013.igem.org/KillSwitch

References